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>>>>> Help ME Circle <<<< >>>> 16 July 2011 <<<<
Editorship : j.van.roijen@chello.nl
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Reference:

*Infectious XMLV in Human Cultures from
Mouse Xenografts* - Help ME circle, 9 July
2011 - Co-Cure: http://bit.ly/ncSEon


~jvr


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http://bit.ly/nCVJUH


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When Science Journals are
Scarier than Science Fiction


By Kent Heckenlively, Esq.


My choice for the scariest reading of the year was
recently published in the journal Cancer Biology and
Therapy and has the unwieldly title of *Frequent
Detection of Infectious Xenotropic Leukemia Virus
(XMLV) in Human Cultures Established from Mouse
Xenografts* (http://bit.ly/oONQ5N).

For those of you who may be confused by the idea of
a "xenograft" I'll provide you with the definition given
by the U. S. Public Health Service:

*Any procedure that involves the transplantation,
implantation, or infusion into a human recipient of
either

(a) live cells, tissues, or organs from a non-human
animal source or

(b) human body fluids, cells, tissues or organs that
have had ex vivio contact with live non-human animal
cells, tissues, or organs.*

This covers vaccines as well as other surgical
procedures in which human tissue is manipulated prior
to transplantation.


In the scientific community mouse xenografting is
often used to manipulate cancer cells for research
purposes, among other things.

With research that has linked XMRV (which is a
xenotropic murine leukemia virus) to prostate cancer,
chronic fatigue syndrome/ME, and to a lesser extent
autism, scientists from Johns Hopkins University and
the University of Texas Southwestern Medical Center
as well as a few other institutions thought it made
sense to investigate the frequency of XMLVs in human
cell lines *established from mouse xenografts and to
search for the evidence of horizontal spread to other
cell lines.*

In layman's terms the question they were asking was,
*when we do xenografting with mouse biological
products how often do we get XMLVs popping up in
our samples?*

The answer they found is that six out of twenty three
(26%) mouse DNA free xenografts *were strongly
positive for MLV and their sequences had greater than
99% homology to known MLV strains.*

These samples were obtained from seven independent
laboratories.

Further on the authors wrote:

*Of the 78 non-xenograft derived cell lines maintained
in the xenograft culture containing facilities, 13 (17%)
were positive for MLV, including XMRV, a virus strain
first identified in human tissues.* (My daughter with
autism has tested positive for XMRV.) In scientific
terms this is an absolute train wreck.

This means that every surgical patient receiving any
biological product which used mouse tissue in any way
has a one in four chance of being exposed to an
XMLV.

And that also means that any biological sample which
is maintained in a facility containing xenograft cultures
has a 17% chance of becoming infected.

On the question of vaccines, let's just say that only
10% of the nearly 40 vaccines children are expected
to receive prior to the age of five contain mouse
biological products. This translates into roughly a
100% chance that our current generation of children
will be exposed to an XMLV through a vaccination.

Are you scared yet?

It gets worse.

From the article is the following passage:

*In one case XMRV virus infection to a non-xenograft
colorectal carcinoma cell line RKO was demonstrated
from an XMRV containing prostate xenograft derived
cell line 221 Rv1 even though the two cell lines had
been maintained in the same culture facility for only a
few days.*

The translation for a non-medical person is this:

XMRV is highly infectious and spreads easily.

How about this for scary?

*Provirus integration into the genome is not random,
and occurs preferentially at transcription start sites,
CpG islands, DNase-hypersensitive sites and gene
dense regions, suggesting that provirus integration
may influence transcription in the host cell.*

For those of you keeping score at home, the CpG
islands are responsible for methylation. Is any of this
starting to sound familiar? And the transcription start
sites of genes? Pretty damned important.

I know there are those who question the role of
viruses in conditions like autism and state correctly
that a well-functioning immune system will deal with
any such pathogens.

I agree.

The problem is that we don't know the immune status
of people in the population. For example, the
discovery of XMRV itself was preceded by the finding
that men with the most aggressive forms of prostate
cancer had a deficiency in their RNasel gene, lowering
the amount of an anti-viral defense enzyme their body
produced.

How widely is that RNasel mutation spread throughout
the population? Do you know the RNasel status of
your genes? Of your children? And that's just what
we know about.

And can toxic chemicals and heavy metals interfere
with your body's immune system response to
pathogens, regardless of your genetic make-up?

You betcha.

The closest example I can make is that of what
happened to the native population in the Americas
when Europeans crossed the ocean.

A natural barrier was breached and the native
population had no immunity to our pathogens. Some
experts speculate that the diesases Europeans brough
to the New World killed 90% of the native population.

While we have shared the Earth with mice and other
creatures from the dawn of history, we haven't been
culturing their cells, then injecting those cells into our
bloodstream.

We have breached a natural barrier and we need to
ask the question of what consequences have been
wrought from this decision.

In the conclusion the authors write:

*Thus laboratories handling or culturing human
xenografts should monitor for the presence of MLV,
and should consider monitoring personnel for viral
antigens or antibodies to them.

Laboratories working with xenograft cultures should
have full knowledge and understanding of the
potential biological and biohazardous risks and should
not distribute or publish their findings without full
disclosure of the virus status of their
xenograft-derived materials.*

I've heard some commentators lament what it will
take for the medical authorities to deal with this issue
seriously.

They've noted with the despair the finding of XMRV in
men with prostate cancer, those with chronic fatigue
syndrome/ME and children with autism and how it has
failed to provoke action.

They've said to me:

"These people have no problem screwing over the old
men, those with CFS/ME, and even the children."

With this recent finding regarding the dangers of
XMLV and XMRV transmission in labs, the question
becomes,

*Will these same medical authorities screw the very
people who work for them?*




Kent Heckenlively is a Contributing Editor to Age of
Autism









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